Abstract

Fatty acids from pentanoic acid to tricosanoic acid have been separated on RP-18 plates with and without a concentrating zone. Although similar separation factors, α , were obtained for the acids on both types of plate, visual inspection of the chromatograms revealed that better separation of the acids was obtained on RP-18 plates with concentrating zone, because compact chromatographic bands were obtained. The resolution, R S , values calculated confirmed these observations. Topological indexes based on the distance matrix, the adjacency matrix, information theory, and electrotopological states were calculated for the acids. It was found that R M values were related to topological indexes by linear or quadratic functions.

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