Abstract
The platelet-rich plasma (PRP) has become an attractive topic for soft tissue healing therapy recently. While some clinical reports revealed the effective treatments for knee osteoarthritis, lateral epicondylitis, and rotator cuff tears, other case studies showed that there was no statistically significant healing improvement. The efficacy of the PRP therapy is still unclear clinically. Thus, a significant amount of basic studies should be conducted to optimize the preparation procedure and the platelet concentration of the PRP. In this work, a 3-chamber co-culture device was developed for the PRP study in order to reduce the usage of primary cells and to avoid the PRP gelation effect. The device was a culture, well partitioning into 3 sub-chambers. Tenocytes and PRP could be respectively loaded into the sub-chambers and co-cultured under the interlinked medium. The results showed that a higher platelet number in the PRP could diffuse higher concentration of the growth factors in the medium and induce higher tenocyte proliferation. The 3-chamber co-culture device provides a simple and practical tool for the PRP study. It is potentially applied for optimizing the preparation procedure and platelet concentration of the PRP therapy.
Highlights
In the research of sports medicine, the use of platelet-rich plasma (PRP) has recently become an attractive approach for stimulating and accelerating the soft tissue healing process [1,2]
As the tissue healing process is stimulated by the platelet-derived growth factors, the platelet number of the PRP is expected to have correlation with the concentration of the growth factor
It is observed that when the whole blood had a lower platelet number, the PRP contained lesser platelets after centrifugation
Summary
In the research of sports medicine, the use of platelet-rich plasma (PRP) has recently become an attractive approach for stimulating and accelerating the soft tissue healing process [1,2]. The PRP is prepared by centrifuging whole blood drawn from a patient. This centrifugation process can concentrate the platelets and remove the blood cells. As the platelets contain hundreds of growth factors, which are very important in the healing process, the PRP with many more platelets than whole blood may encourage the healing response. As long as the plasma has a greater concentration of platelets than whole blood, this plasma is called PRP. The PRP with 3–5 times platelet concentration can be used for the tissue injection where healing is desired
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