Abstract
Certain genes from the glutathione S-transferase superfamily have been associated with several cancer types. It was the objective of this study to determine whether alleles of the glutathione S-transferase zeta 1 (GSTZ1) gene are associated with the development of sporadic breast cancer. DNA samples obtained from a Caucasian population affected by breast cancer and a control population, matched for age and ethnicity, were genotyped for a polymorphism of the GSTZ1 gene. After PCR, alleles were identified by restriction enzyme digestion and results analysed by chi-square and CLUMP analysis. Chi-squared analysis gave a chi2 value of 4.77 (three degrees of freedom) with P = 0.19, and CLUMP analysis gave a T1 value of 9.02 with P = 0.45 for genotype frequencies and a T1 value of 4.77 with P = 0.19 for allele frequencies. Statistical analysis indicates that there is no association of the GSTZ1 variant and hence the gene does not appear to play a significant role in the development of sporadic breast cancer.
Highlights
Breast cancer is the most common malignancy in women in the Western world, with an incidence approaching one in in the USA in 1980 [1] and one in in Australia in 1991 [2]
GSTP1 has a polymorphism resulting in an amino acid change from isoleucine to valine, which may predispose to certain cancers, including breast cancer, and has variously bp = base pairs; GST = glutathione S-transferase; GSTZ1 = glutathione S-transferase zeta 1; PCR = polymerase chain reaction
The purpose of the present study is to investigate the role of the GSTZ1 gene in the development of sporadic breast cancer
Summary
Breast cancer is the most common malignancy in women in the Western world, with an incidence approaching one in in the USA in 1980 [1] and one in in Australia in 1991 [2]. Carcinogens in the body are detoxified by specific enzymes that aid in interception and removal from the cell or in modification involving addition of chemical residues to the reactive sites that cause DNA damage, allowing safe storage, prior to removal [4] Among these enzymes are the glutathione S-transferase (GST) superfamily of enzymes, which prevent the action of electrophilic and alkylating carcinogens by binding to glutathione [5]. Certain genes from the glutathione S-transferase superfamily have been associated with several cancer types It was the objective of this study to determine whether alleles of the glutathione S-transferase zeta 1 (GSTZ1) gene are associated with the development of sporadic breast cancer. Conclusion: Statistical analysis indicates that there is no association of the GSTZ1 variant and the gene does not appear to play a significant role in the development of sporadic breast cancer
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