Investigation of genotype and allele distribution of ppargc1a-t19c locus in holstein cattle

Abstract

This study aimed to investigate the genotype and allele distribution of the PPARGC1A-T19C locus in Holstein cattle. The PPARGC1A gene plays a crucial role in significant metabolic processes such as adaptive thermogenesis, gluconeogenesis, skeletal muscle fiber type switching, mitochondrial biogenesis, and adipogenesis. Additionally, it holds a key role in the activation of many hormone receptors and transcription factors, thereby emerging as a potential candidate gene for the milk traits of cattle. In this study, genotyping was performed using the PCR-RFLP method on 50 Holstein animal samples. The 205 bp PCR product was digested with the BsuRI restriction enzyme to reveal genotypic differentiation. Two different genotypes, TC and CC, were identified. The genotype frequencies were found to be 0.30 (TC) and 0.70 (CC). Allele frequencies were determined as 0.15 (T) and 0.85 (C). The Chi-square test showed that the PPARGC1A-T19C locus distribution was in Hardy-Weinberg equilibrium (p>0.05). In conclusion, these findings can be utilized to understand intra-breed genetic diversity and develop more effective selection strategies in the genetic improvement programs of the breed.