Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis

Yun Zhu,Peng Li,Yuangang Shi,Yun Ma,Xiaolong Kang,Liyun Han,Xingang Dan

doi:10.1038/s41598-021-91101-w

Yun Zhu, Peng Li + Show 5 more

Open Access

https://doi.org/10.1038/s41598-021-91101-w

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Abstract

ulked Segregant Analysis (BSA) is a rapid strategy for identifying genetic markers in specific regions of the phenotypical population and it has been widely used for QTLs mapping in smaller mixed F2 and F3 populations. We applied a modified BSA method to assessed genome-wide homozygous and heterozygous linkage patterns in the Chinese Wagyu Beef Cattle F2/F3 mixed population. Two overlapped regions from F2 and F3 populations on autosomes were found with high-density heterozygote alleles between high and low intramuscular fat groups. Regions from 24.8 M ~ 29.6 M of chromosome 23 were identified as most significantly correlated to the intramuscular fat in our samples. We also identified other 4 potential loci on chromosomes 5, 9, 15, and 21 correlated with Intramuscular fat. This study provided a novel low-cost method for QTLs mapping and identify molecular markers of phenotypical changes in a small mixed population.

Highlights

Ulked Segregant Analysis (BSA) is a rapid strategy for identifying genetic markers in specific regions of the phenotypical population and it has been widely used for QTLs mapping in smaller mixed F2 and F3 populations
This study introduced the frozen sperm of Japanese Wagyu cattle hybridized with Qinchuan and Luxi cattle in order to improve the potential of the quality of meat
Intramuscular fat (IMF) of meat is distributed in the form of white marbling, commonly known as marbling, and is one of the important indicators for meat quality

Summary

Introduction

Ulked Segregant Analysis (BSA) is a rapid strategy for identifying genetic markers in specific regions of the phenotypical population and it has been widely used for QTLs mapping in smaller mixed F2 and F3 populations. This study introduced the frozen sperm of Japanese Wagyu cattle hybridized with Qinchuan and Luxi cattle in order to improve the potential of the quality of meat. The word “marbling” is defined by the amount and distribution of visible IMF that accumulated within the muscle and muscle fiber bundles It is an important economic trait in all cattle and stands out in the Japanese cattle[1]. Qinchuan and Luxi cattle are the local breeds in China They are both steer, less intramuscular fat deposition in comparison with Japanese wagyu cattle, Qinchuan and Luxi cattle have smaller transection area of the longissimus dorsi muscle and lower IMF content. Japanese wagyu cattle are being brought in to cross-breed with local Chinese cattle in the hope of improving the quality of meat.

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