Abstract
Pasteurella multocida causes fowl cholera which is an economically important disease in poultry industries around the world. In this study, we analyzed the capsular genotype, lipopolysaccharide (LPS) genotype, virulence-associated genes (VAGs) patterns, antimicrobial resistance and genetic diversity in a total of 9 P. multocida isolates from poultry with fowl cholera between 2014 and 2019 in Korea. When combining the capsular types with the LPS genotypes, two isolates of the 9 isolates were A:L3, and the others were non-typeable (NT): L3. Of the 23 VAGs, all the isolates harbored ptfA, fimA, hsf-1, hsf-2, pfhA, exbB, exbD, tonB, hgbA, hgbB, fur, sodA, sodC, pmHAS, ompA, ompH, oma87, plpB, psl, and nanH, whereas toxA gene was not detected in any of the 9 isolates. In addition, among the 11 antimicrobials, most of the isolates except for one isolate resistant to florfenicol, exhibited susceptibility to all the antimicrobials. Multi-locus sequence typing (MLST) analysis revealed 5 different sequence types (ST): ST8, ST351, ST352, ST353, and ST368. The ST351, ST352, ST353, and ST368 were identified for the first time in this study, and ST352 and ST353 isolates were largely prevalent nationwide. These STs isolates should be monitored continuously because in some cases, ST352 and ST353 isolates demonstrated high mortality rates. Although only limited numbers of isolates have been analyzed, our findings provide overall characteristics and epidemiological information of the P. multocida strains recently prevalent in Korea.
Highlights
Fowl cholera is an acute and fatal septicemic disease that can affect all types of birds and causes significant economic losses in poultry industries around the world
The capsular types of the 9 isolates were determined by capsular PCR typing and it was confirmed that two isolates (18PM2 and 19PM1) were capsular type A, but the others were non-typable (Supplementary figure S1)
The transmission electron microscopy (TEM) image of cells of 17PM1, a representative non-capsulated isolate, was presented in figure 1A and these cells were not covered with a layer of ferritin granules
Summary
Fowl cholera is an acute and fatal septicemic disease that can affect all types of birds and causes significant economic losses in poultry industries around the world. Pasteurella multocida (P. multocida) has been reported to be the causal agent of fowl cholera (Li et al, 2018). Capsular type A is the most prevalent serovar in cases of fowl cholera (Swayne, 2020), other capsular types are occasionally incriminated, type F in turkeys, and in some avian strains, non-capsular types have been reported (Davies et al, 2003). In Korea, previous studies suggest that capsular type A has been prevalent in fowl cholera (Kwon & Kang, 2003; Woo & Kim, 2006; Kim et al, 2011); these studies provide information only before 2006, eRBCA-2020-1390
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
