Abstract
We have developed two techniques to investigate the aggregation structure of monolayers at the air-water interface: a reflectance-measuring technique and observation by using a phase contrast microscope. Using these techniques, we investigated the aggregation structure of fatty acid (C 12C 24) monolayers at the air-water interface without using fluorescent probes. From the reflectance measurement, it is suggested that the aggregrates (domains) grow even below 0.1 mN m , and the size of the domains in gaseous states increases with the alkyl-chain length from lauric acid (C 12) to arachidic acid (C 20), but it decreases abruptly in behenic acid (C 22). We found that the aggregation structure of monolayers at the air-water interface can be visualized using a phase contrast microscope. Using this technique, we first observed the domain morphology in fatty acid (C 14C 24) monolayers directly and in real-time without adding fluorescent probes. The results suggested by the reflectance measurement were directly confirmed by observation using a phase contrast microscope.
Published Version
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