Abstract
Ralstonia sp. TSC1 previously isolated from cowpea nodules was tagged with gfp gene by transposon insertion. The resultant gfp-tagged Ralstonia sp. TSC1 showed no difference in physiological properties such as growth rate, exopolysaccharide formation and colony morphology. When it was co-inoculated with compatible bradyrhizobial strains, the observation of fluorescence microscopy showed that TSC1 strains were colonized in subsurface of cowpea nodules and stems. In addition, TSC1 positively and negatively modulated symbiotic performance with the bradyrhizobia in terms of nodulation and symbiotic nitrogen fixation. The results suggested that Ralstonia sp. TSC1 is an endophyte with beneficial, neutral or detrimental effects on cowpea plants when in presence of effective bradyrhizobia. Key words: Ralstonia sp., cowpea, endophyte, green fluorescent protein,bradyrhizobium, nodulation.
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