Abstract
miRNAs have been proven to be highly potential biomarkers for a variety of cancers and cardiovascular diseases. Typically miRNA detection method uses polymerase chain reaction (PCR) to amplify the miRNA concentration. This method has the disadvantage of being time-consuming and unable to directly detect the original concentration. Therefore, this study intends to design a FET biosensor to detect miRNA. In order to design a stable and highly sensitive miRNA FET biosensor, we used different concentrations of ssDNA probe to functionalize the sensor chip to detect target miRNA concentration, to compare the dynamic range and sensitivity. Find out the best condition to make a miRNA FET biosensor with high sensitivity, high stability and large dynamic range.
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