Abstract
A characteristic common to herpesviruses is the ability to establish a latent infection in the hosts, a transcriptionally active region has detected during latency as well as a set of RNA that are known as Latency Associated Transcripts (LATs), their functions have been clarified in recent work. The present work was carried using different bioinformatics method in order to determine if Herpesvirus Canine 1 (CHV-1) has a region associated with latency. Our result was the selection of nine sequences candidate of micro RNA (miRNA) (MIREval 2.0 software), and 26 miRNA (miRNAFold v.1.0 software), of them, were selected 14 with real precursors of miRNA, two were found between the RL2 and RS1 genes, one in the RL2 gene and 11 in the RS1 gene. The results showed that the similarities of these regions are very low among the herpesviruses analyzed, so it was not possible to deduce the presence of the LAT gene in canine herpesvirus type 1 with bioinformatics. On the other hand, the comparison showed that the miRNA predicted: chv1-mir-mirnafold-8 has similarity with the ebv-mir-BART7-3p of Epstein-Barr Virus (EBV), in this way, the microRNAs predicted by means of bioinformatic programs met the theoretical requirements of these molecules, however at not having a degree of preservation in other herpesviruses, the expression by CHV-1 in latency cannot be confirmed and it is necessary to identify through experimental tests.
Highlights
Herpesviruses vary in the range of hosts, genome size and molecular composition, they share biological feature and have a common structure in the virion
The results showed that the similarities of these regions are very low among the herpesviruses analyzed, so it was not possible to deduce the presence of the Latency Associated Transcripts (LATs) gene in canine herpesvirus type 1 with bioinformatics
Since the LAT gene is transcribed into several microRNAs, when these mRNAs were identified, the probable precursor region of CHV (LAT) is identified, for this analysis, two programs were used in sequence MIREval and CHV-1 strain V777
Summary
Herpesviruses vary in the range of hosts, genome size and molecular composition, they share biological feature and have a common structure in the virion. Carnivorous herpesviruses are represented within the same subfamily (Alphaherpesvirinae) by three phylogenetically closely related viruses: CHV-1, Herpesvirus Feline 1 (FeHV-1) and Phocide Herpesvirus PhHV-1 [1], they have a 51% homology in their genomes [2]. Each region is flanked by DNA inverted repeat sequences (TRL/IRL and TRS/IRS) [3] [4]. There is a group of “central genes” that are conserved among alpha, beta and gamma subfamilies since they are genes that encode proteins related to viral entry and replication [6]. CHV-1 contains 76 Open Reading Frame (ORFs) that encode for the same number of functional proteins. 61 are located in the UL region, seven in the US and four repeated in the terminal repetitions (TRS) and internal repetitions (IRS) [5]
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