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Abstract
Pristine and engineered metal nanoparticles are widely applied in various fields of industry, and as consequences, they are useful as well as harmful to human health and environment. This study aimed at synthesizing the green zinc oxide nanoparticles (ZnNPs) using the leaf extract of Ocimum sanctum Linn and assessing its toxicity on human skin epidermal (HaCaT) and human lung epithelial (A549) cells. The synthesized green ZnNPs (gZnNPs) were characterized by using dynamic light scattering (DLS) and a high-resolution transmission electron microscope. The average size of gZnNPs obtained was 62 nm with a spherical shape. The effects of gZnNPs on the viability of HaCaT and A549 cells were investigated using tetrazolium salt (MTT) for 24 h. We have seen more reduction of cell viability of A549 cells in comparison to HaCaT cells. The induction of intracellular reactive oxygen species (ROS) was measured using DCFDA assay and showed a slightly high intensity of green fluorescence in A549 than HaCaT cells. The different oxidative stress biomarkers such as ROS generation and lipid peroxide were increased, and GSH was decreased in a dose-dependent manner. The apoptotic and necrotic effect of gZnNPs in both cells was carried out using Annexin-V-FITC and propidium iodide staining. More apoptotic and necrotic cells were found at a higher concentration of gZnNPs exposure. Also, we determined the effect of gZnNPs at the molecular level by evaluating the apoptotic and inflammatory markers, in which gZnNPs downregulated Bcl2 and upregulated Bax, caspase-3, and TNF-α in HaCaT and A549 cells. Ultimately, gZnNPs exerted toxicity and apoptosis in HaCaT and A549 cells.
Highlights
Engineered nanoparticles are an inventive class of materials
zinc oxide nanoparticles (ZnNPs) was synthesized using the extract of Ocimum leaves and used to explore the potential influence of exposure to ZnNPs on the skin and pulmonary inflammation in HaCaT and A549 cells, respectively
TEM analysis explored that the average size of synthesized ZnONPs was 58:5 ± 2:5 nm with a facecentered cubic structure with a spherical shape (Figure 1)
Summary
Engineered nanoparticles are an inventive class of materials. Due to this reason, development of nanotechnology is used in different interdisciplinary areas, e.g., medical purposes and industry. The improvement of nanotechnology headed to the productions of nanoparticles (NPs), as contrast agents or in targeted treatments Due to their more applications in clinical purposes, the NPs are intensively investigated, and more development was done on new techniques to biological synthesize eco-friendly nanomaterials and on assessing their biological effects on living organisms [1]. In. Oxidative Medicine and Cellular Longevity the current investigation, the leaf extracts of Ocimum sanctum were used to biologically synthesize of ZnONPs and mediate its toxicity involved various mechanisms, in particular the production of excess ROS. Oxidative Medicine and Cellular Longevity the current investigation, the leaf extracts of Ocimum sanctum were used to biologically synthesize of ZnONPs and mediate its toxicity involved various mechanisms, in particular the production of excess ROS As it is well known, mitochondrial dysfunction is the major source of ROS overload [5]. In this study, we will investigate the toxic effects of gZnNPs on HaCaT and A549 cells
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