Abstract
Cyclodextrin glucanotransferase (CGTase) production by free and calcium alginate-immobilized cells was investigated in an airlift reactor. The average volumetric productivity with continuous fermentation (13.65 U ml −1 h −1) was found to be greater than the free cells (6.89 U ml −1 h −1). The total CGTase enzyme produced with continuous fermentation was found to be about 98% greater than with that of free cells. The immobilized Bacillus particles showed a half-life of 18 days during continuous fermentation under airlift conditions.
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