Investigation of connexin gene expression patterns by in situ hybridization techniques.

Abstract

AbstractThe principle of in situ hybridization is the specific annealing of a labeled DNA or RNA probe to complementary sequences present in tissues, followed by detection of the probe. The complementary sequences can be present in chromosomal DNA, viral DNA or RNA, transcript RNA, etc. A particular in situ hybridization technique corresponds to each type of these nucleic acids. All these techniques, including in situ hybridization to transcript RNA, have been described in detail in several books or specialized papers that the reader is invited to consult (e.g., 1–6). This chapter deals only with in situ hybridization to transcript RNA, and it is focused on the localization of connexin (Cx) gene transcripts in mammalian tissues. In the text below the expression “in situ hybridization” is used only in the restrictive sense of “in situ hybridization to transcript RNA”.KeywordsHybridization TechniqueBidistilled WaterFixation SolutionSaline Sodium CitrateTranscription AssayThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.