Abstract
The aim of this study was to analyze the distribution of chlorin type photosensitizers (PS): chlorin e6 (Ce6) and dimethyl ester of chlorin e 6 (DME), in human adenocarcinoma HT29 monolayer and multicellular spheroid cell cultures. There is an assumption, that the chemical modification of Ce6 molecules causes a change of intracellular location and the enhanced photosensitizing activity. Indeed, photodynamic therapy on monolayer cell culture with DME showed two times higher photokilling ability comparing with that of non-modified analogue. Ce6 and DME biodistribution processes in tumor tissue were studied on multicellular tumor spheroids model. Total amount of DME in multicellular tumor spheroids exceeded 1,3 times accumulation of Ce6. According to fluorescence microscopy studies, Ce6 and DME distribution patterns in the spheroids bulk were similar. Application of 2D and 3D tumor models for the analysis of photosensitizer distribution may allow predicting the photosensitizer biodistribution features for photodynamic therapy in vivo.
Highlights
The aim of this study was to analyze the distribution of chlorin type photosensitizers (PS): chlorin e6 (Ce6) and dimethyl ester of chlorin e6 (DME), in human adenocarcinoma HT29 monolayer and multicellular spheroid cell cultures
После трипсинизации клеток монослоя/сфероидов накопление ФС оценивали с использованием проточного цитометра FACSCalibur (Becton Dickinson, США), оснащенного аргоновым лазером с длиной волны возбуждения 488 нм (20 мВ)
Zorin V.P. et al Intra- and intermembrane distribution of chlorin e6 derivatives // Proceedings SPIE
Summary
Зорин Исследование распределения хлориновых фотосенсибилизаторов в двухмерных и трехмерных клеточных культурах. Прохорова РАН, Москва, Россия 3Белорусский государственный университет, Минск, Республика Беларусь 4Centre de Recherche en Automatique de Nancy, CNRS, Université de Lorraine, Nancy, France 5Institut de Cancérologie de Lorraine, Vandoeuvre-lès-Nancy, France 6Международный государственный экологический институт им. Цель данного исследования заключалась в оценке распределения хлориновых фотосенсибилизаторов – хлорина е6 (Ce6) и его диметилового эфира (DME) – в клетках НТ29 аденокарциномы человека в моделях монослоя клеток и мультиклеточных сфероидах. DME накапливается в 2,3 раза более эффективно в клетках монослоя по сравнению с Ce6. Для цитирования: Фаррахова Д.С., Яковец И.В., Лощенов В.Б., Bolotine L.N., Зорин В.П., Исследование распределения хлориновых фотосенсибилизаторов в двухмерных и трехмерных клеточных культурах // Biomedical Photonics – 2017.
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