Investigation of Chitosan Nanoparticles Loaded with Protocatechuic Acid (PCA) for the Resistance of Pyricularia oryzae Fungus against Rice Blast.

The Pham,Cuu Nguyen,Dai Nguyen,Tien Le,Duy Nguyen,Thuan Thi,Trong Nguyen,Do Vo,Thi Nguyen,Thanh-Truc Nguyen,Long Bach

doi:10.3390/polym11010177

Abstract

In this study, chitosan nanoparticles were used as a carrier for Protocatechuic acid (PCA) to resist Pyricularia oryzae against rice blast. The final compound was characterized using zeta potentials for its surface electricity, Fourier transform infrared (FT-IR) analysis and transmission electron microscopy (TEM) were conducted for functional groups and for particle sizes and shape, respectively. The zeta potential results showed that loading PCA causes chitosan nanoparticle (CSNP) to decrease in surface electrons. The TEM images revealed that the particle size of chitosan (CS), although increasing in size when carrying PCA molecules, showed sufficient size for reasonable penetration into fungal cells. The FT-IR analysis showed that all functional group in CSNP carried PCA matched with previous studies. The antifungal test showed that diameters of inhibition zone of CS increases significantly after loading PCA, exhibiting the strongest antimicrobial effect on the Pyricularia oryzae fungus compared with weaker effects exhibited by CSNP alone or PCA. Our results suggested that CSNP loaded with PCA could be a potential compound for eradication of Pyricularia oryzae and that further testing on in vitro rice plants is recommended to reaffirm this possibility.

Highlights

The rice blast caused by fungi Pyricularia oryzae has a long research history in agriculture.For centuries, this disease has been observed in many Asian countries such as Japan, India, China, and Vietnam
Giventhe thesuperior superiorfeatures featuresand andhigh highbiocompatibility biocompatibility described described above, above, this this study study aims aims to to overcome the disadvantages of the compound and alleviate the adverse effect of Pyricularia oryzae overcome the disadvantages of the compound and alleviate the adverse effect of Pyricularia oryzae on on attempting synthesisofofa acompound compoundconsisting consistingof of chitosan nanoparticle (CSNP)
The results showed that both Protocatechuic acid (PCA), CSNP and CS@PCA exhibited anti-fungal properties

Summary

Introduction

The rice blast caused by fungi Pyricularia oryzae has a long research history in agriculture. This disease has been observed in many Asian countries such as Japan, India, China, and Vietnam. Blast disease has been reported in over countries around the world [1]. Farmer usually use pesticides with high concentration of harmful chemicals. This approach is not effective and could impair the quality of the rice. Nano-pesticide has been reported to provide efficient alternatives for antifungal management in agriculture without harming the environment [3,4]

Objectives

Results

Conclusion