Abstract
This study is to investigate Brucellosis, Toxoplasmosis and Foot and Mouth disease of buffalo herds in Baghdad villages. 114 samples (serum) were collected, including 22 for calves less than 6 months and 92 samples of females aged between three to seven years, the samples were collected by staff of Baghdad Veterinary Hospital / Veterinary Directorate, and has been working in Central Veterinary Laboratory/ Veterinary Directorate.
 The ELISA diagnostic kits are selected for the detection of antibodies of Brucella abortus, Toxoplasmosis and Foot and Mouth disease (Multispecies). 
 The result of the research is the presence of different levels of infection in the same herd
 58.18% of Brucella abortus, 11.40% of Toxoplasmosis and 44.73 % of Foot and Mouth disease (Multispecies).
 The purpose of the study is to investigate the three diseases and their compatibility with the symptoms, although buffalo herds have not been previously vaccinated against the diseases, which proves the existence of different levels of infection in one herd and it adversely affect the development of livestock.
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