Abstract
Bufavirus (BuV) and human parvovirus 4 (PARV4) belong to the Parvoviridae family. We assessed BuV and PARV4 DNA presence by real-time PCR analysis in stool, blood and respiratory samples collected in patients from Marseille and Nice, two large cities in the South-East of France. Bu-V DNA was detected in diarrheic stool samples from 92 patients (3.6% of 2583 patients), particularly men and adults, and patients from the nephrology and the infectious disease departments. Among the patients with a BuV-positive stool sample and for whom at least one blood sample was available (n = 30 patients), BuV DNA was detected also in 3 blood samples. In contrast, BuV DNA was not detected in any of the respiratory samples from 23 patients with BuV-positive stool. BuV detection rate was comparable in stool samples from patients with and without diarrhea. We did not detect PARV4 DNA in any of the stool specimens (n = 2583 patients). Our results suggest that PARV4 fecal–oral transmission is rare or non-existent in the South-East of France while BuV circulates with a relatively high rate in this area.
Highlights
Viruses of the Parvoviridae family are small single-stranded DNA viruses that infect a wide range of organisms, from invertebrates to humans
BuV DNA has been detected in stool samples, mostly from patients presenting with diarrhea, in many countries
The aims of the present study were (i) to investigate BuV and parvovirus 4 (PARV4) presence in stool samples from patients with diarrhea in the South-East of France; (ii) to check whether in patients with positive stools, both viruses can be found in blood and respiratory samples; (iii) to determine whether the detection rate of these viruses varies in patients with and without diarrhea in hospital settings
Summary
Viruses of the Parvoviridae family are small single-stranded DNA viruses that infect a wide range of organisms, from invertebrates to humans. The first human parvovirus was discovered in 1965 and since 2005, many new parvoviruses have been identified in human samples [1,2]. Bufavirus (BuV), a virus from the genus Protoparvovirus, was identified in 2012 from fecal samples of children with diarrhea in Burkina Faso [3,4]. BuV DNA has been detected in stool samples (prevalence ranging from 0 to 4%), mostly from patients presenting with diarrhea, in many countries. The causative role of BuV in gastroenteritis remains unclear, and its association with other clinical presentations remains largely to be explored [5,6,8]
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