Abstract
This study has investigated bovine coronavirus (BCoV) and bovine rotavirus (BRV), which are among the most important causes of diarrhea in calves leading to financial losses in Turkey and all over the world BCoV and BRV were detected by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), which is one of the most reliable method of diagnosis, The results obtained by RT-PCR were compared to the sensitivity of the commercial Rota-Corona Rapid Test Kits used by clinical veterinarians in fields. In this study, 96 fecal samples were examined from diarrheic calves in cattle farms in the cities of Konya and Afyon for BRV and BCoV firstly by BoviD-5 Ag rapid test kit, and then we applied the RT-PCR test. A comparison of the rapid test kit with the RT-PCR in terms of sensitivity and specificity revealed the 83% sensitivity and 100% specificity of the BRV and 7.6% sensitivity and 100% specificity of BCoV. In conclusion the practical and rapid diagnosis of the disease using of Rapid Diagnosis kit used by the clinician veterinarians may be useful, but the results must be interpreted with caution since the sensitivity of the test decreases due to the reduction in the number of viruses in the later stages of the infection. .
Highlights
This study has investigated bovine coronavirus (BCoV) and bovine rotavirus (BRV), which are among the most important causes of diarrhea in calves leading to financial losses in Turkey and all over the world BCoV and BRV were detected by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), which is one of the most reliable method of diagnosis, The results obtained by RT-PCR were compared to the sensitivity of the commercial Rota-Corona Rapid Test Kits used by clinical veterinarians in fields
One swab taken out of the rectum was put in the solution in the rapid test kit, and another was homogenized by putting it into Phosphate Buffer Saline (PBS), and stored at -20oC until it was used for RT-PCR
We examined 96 diarrheic fecal samples in total for BCoV and BRV by rapid diagnostic test and RT-PCR method
Summary
Neonatal calf diarrhea caused by viral, bacterial and and coronavirus in the cases of calf diarrhea have been protozoon agents is one of the infections characterized found to reach up to 50% and 80% respectively. By enteritis leading to weight loss and deaths in calves Rotaviruses generally cause infections characterized by under one month of age (Murphy et al.,1999). Through the feces of the infected animals, a high level of viral particles (approximately 1011 particle/g) is shed around. This shedding reaches the highest level on the third and fourth days, and the virus can survive in the feces for several months (Murphy et al, 1999)
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