Abstract
Background and Objectives:Occupational exposure to silica dust can lead to biochemical damage. Malondialdehyde (MDA) can be considered as a primary marker for measuring the level of oxidative stress in a living organism. This study was conducted in order to evaluate the level of MDA in blood and urine of mice exposed to silica dust.Material and Methods:In this experimental study, 72 mice (BALB/c) were randomly allocated to five exposed groups and 1 control group. Exposure of mice to pure 99% silica dust was done in closed containers. Blood sampling was performed from the heart of mice and urine sampling fulfilled by insertion into a metabolic cage. The RAOet al. method was used to measure MDA.Results:The highest level of plasma MDA in group 1 in the 4thmonth was 8.4±0.41 nmol/l and the lowest level of MDA was 1.3±0.2 nmol/l in the third sampling in the control group, also the highest amount of urine MDA in the first and second groups and 4 months after exposure was 1.16±0.51 nmol/l, and the lowest in the control group and in the third sampling was 0.48±0.06 nmol/l. A significant difference was found between the levels of MDA in all exposed groups at different times except for the 5thgroup with the lowest concentration (P< 0.05).Conclusion:MDA in blood and urine could be proposed as a good biomarker for the evaluation of biochemical damages caused by silica dust. Measuring MDA is also a simple and inexpensive method that does not require complex equipment and can be used as an early detection test for biochemical damages caused by silica.
Highlights
Silica is the most common mineral on earth and is one of the materials used in various mineral industries such as casting, glass processing, tile and ceramic factories, cleaners, supply of various chemicals and glazes, as well as in open and underground mines [1]
MDA in blood and urine could be proposed as a good biomarker for the evaluation of biochemical damages caused by silica dust
The Open Biochemistry Journal, 2019, Volume 13 33 kers exposed to crystalline silica, a significant difference was observed between the levels of Malondialdehyde (MDA) in workers exposed to silica compared to the control group [6 - 8]
Summary
Silica is the most common mineral on earth and is one of the materials used in various mineral industries such as casting, glass processing, tile and ceramic factories, cleaners, supply of various chemicals and glazes, as well as in open and underground mines [1]. One of the damages caused by silica is biochemical damage that is studied by measuring biochemical indicators in the blood or urine. Aldehydes are produced from the oxidation of phospholipids in a cell membrane [10], and MDA is a secondary compound formed by the peroxidation of unsaturated fatty acids in the body that can be used as a marker to measure the level of oxidative stress in a living organism. Malondialdehyde (MDA) can be considered as a primary marker for measuring the level of oxidative stress in a living organism. This study was conducted in order to evaluate the level of MDA in blood and urine of mice exposed to silica dust
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