Investigation of Biofilm Forming Capabilities on Stainless Steel Surfaces of Some Foodborne Pathogens

Abstract

Biofilm is a major problem in the food industry produced by pathogenic microorganisms, because adhered cells to biotic or abiotic surfaces are more resistant against antimicrobials and disinfectants than planktonic cells.The retention of bacteria on food contact surfaces is an important in food processing, catering and the domestic environments. Salmonella species and Listeria monocytogenes are the most important food‐borne pathogenic bacteria. In this study, L. monocytogenesis and Salmonella species isolated from several food sources in the region of Eskisehir were screened for their biofilm forming ability on the stainless steel material and biofilm development stages were examined with scanning electron microscopy (SEM) In our study, L.monocytogenesis ve Salmonella spp isolates were identified by standard microbiological procedures. The biofilm forming capabilities of isolates were tested by microtiter plate and tube methods. Moderate and strong degree of biofilm‐forming organisms on the stainless stain surfaces were taken into electron microscopic procedures. They were examined on 2, 4, 6, 24 ve 48 th hours for their structure of the biofilm and imaged by SEM (JEOL JSM‐5600LV). Stainles steel materials are commonly used surfaces in food production, storage and processing processes. L.monocytogenesis and Salmonella spp . have been shown to adhere to stainless steel surfaces. According to our results, biofilm formation has started from 24 th hour. Isolation of theese microorganisms from variety foods showed that these foods can be easily infected with bacteria at any stages of food production. It carries risks for public health.