Investigation of Biofilm Formation Efficiency in ESβLs of Pathogenic Escherichia coli Isolates

Abstract

Up to 70 clinical Escherichia coli samples were examined, with 30 identified as extended-spectrum β-lactamase (ESβL) isolates. These isolates were gathered from inpatients and outpatients at some Baghdad hospitals. Urine, pus, feces, and blood samples were obtained from government hospital patients of both genders and diverse age groups. Production of ESβL and the quick antibacterial diagnosis was checked using the VITEK2 for susceptibility experimentation and identification of the bacterial sample. The results showed that 30 isolates only in the current study were ESβL producers. Furthermore, all showed biofilm formation after 24 hours of incubation some of these isolates were the ability of biofilm formation decreased after 48 hours compared with negative control. The formation of biofilm has founded a vary from strong to weak. The variation in the bacterial study may depend on their antibiotic resistance and the sample’s source. Therefore, this study considers the need to focus in subsequent studies on the effect of biofilm formation and resistance of isolates, which produce beta-lactamase enzymes for many antibiotics, thus avoiding the occurrence of double health crises for infected patients.