Investigation of Antihyperglycemic Activity of Glycine max (L.) Merr. on Serum Glucose Level in Diabetic Mice

Abstract

Glycine max (Soybean) is being used as fresh, fermented or dried food. Petroleum ether (GM-PEE), alcoholic (GM-ALE) and aqueous extract (GM-AQE) of seeds of Glycine max were prepared and evaluated for antihyperglycemic activity. GM-PEE (100 mg/kg), GM-ALE (100 mg/kg, p.o.), GM-AQE (100, 200 and 400 mg/kg, p.o) and glyburide (10 mg/kg, p.o) were administered orally in alloxan-induced(80 mg/kg, i.v.) diabetic mice. Serum glucose level was estimated after acute and chronic treatment with extracts. Oral glucose tolerance test (OGTT) was performed in GM-AQE pretreated animals. GM-PEE (100 mg/kg) and GM-EE (100 mg/kg) did not reduce serum glucose level. GM-AQE (100, 200 and 400 mg/kg) significantly reduced serum glucose level (antihyperglycemic effect) in acute and chronic studies. The antihyperglycemic effect of GM-AQE (100, 200 and 400 mg/kg) showed onset at the 2nd h; peak effect at the 4th h and the antihyperglycemic effect was sustained till the 24th h. In chronic study, reduction in serum glucose level was observed on the 28th day and also after withdrawal for next 7 days. In OGTT, increased glucose tolerance was observed in GM-AQE (100 and 200 mg/kg) treated non-diabetic as well as diabetic mice. It is concluded that GM-AQE but not GM-PEE and GM-ALE showed antihyperglycemic activity.