Abstract
Since ages, natural products have laid the foundation for the development of promising antimicrobials. With the advent of antimicrobial resistance, the search for effective antimicrobials continues as its shortfall will menace the healthcare system. Natsiatum herpeticum remained the least explored plant despite its ethnopharmacological claims. DNA barcoding was performed to identify and ensure quality control of the plant materials used in the experiment. QToF-MS analysis followed by network pharmacology revealed TNF and IRAK4 to be the two gene targets that can be modulated by the compounds present in the extract. Analysis of potential drug-like compounds using molecular docking (against 1KZN, 2VF5, 2W9S, and 4CJN) and MD simulation suggested compound CPD2 to be the most potent molecule against the bacterial targets. Bacteriostatic activity against E. coli was exhibited by the extract (MIC=50 μg/ml) in the microtiter-plate dilution method. Our results suggest that N. herpeticum not only exhibits potential bacteriostatic activity against E. coli but can also modulate host-immune responses via TNF and IRAK4-associated pathways.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
