Investigation of anti proliferative properties and antioxidant activity of aerial parts ethanolic extract of Hypericum perforatum L. by breast cancer 4T1 cell lines

Abstract

The traditional use of herbal medicine has increased significantly in these recent years. Nowadays it is very desirable to investigate and discover new antibacterial and anticancer agents from natural products and medicinal plants. The natural products obtained from organisms such as medicinal plants (secondary metabolites) are known as a powerful source to supplement therapy and prevention of cancer. The aim of this study was to evaluate the cytotoxic effects of Hypericum perforatum L. Extracts on cancerous cells. Sample were collected And After drying at room temperature, samples extracted by water and ethanol. Mouse breast (4T1) cancer cell lines incubated with different concentrations of ethanol extract for 48 hours and cell growth inhibition was determined using MTT assay. In addition the antioxidant activity of these extract were carried out by using several methods. Results of MTT assay showed strong and dose-dependent inhibition of cancer cell growth by ethanolic extract of Hypericum perforatum L. This extract caused a significant decrease in proliferation of tested cancer cell lines. the experimental finding show that the ethanolic extract of Hypericum perforatum L. possess significant anticancer and antioxidant activity ,justifying the use of these plants in traditional medicine ,which may be developed as phytomedicines.