Abstract
Objective: Probiotics are an important part of the microbiota associated with many diseases. The production of food and food supplements containing probiotic products has increased considerably in recent years, due to their protective effect on health and antagonistic effect against some pathogenic microorganisms. However, it is unknown whether the content of these products has the expected effect. In this study was aimed to investigate the antagonistic effect of Probiotics & Prebiotics® commercial product. 
 Material-Method: The probiotic food supplement was obtained from Aym-Net®. Antagonistic effect of the product on 10 different test bacteria was investigated by the agar spot assay method. Also lyophilized probiotics were cultured in MRS broth medium and centrifuged at 3500 rpm for 25 minutes in order to obtain metabolites that the probiotic microorganisms provide their effectiveness. Effect of probiotic supernatant on test bacteria were determined to disk diffusion test by CLSI. Each test bacteria combined with the probiotic product incubated at 37⸰C for 24 hours to determine the bacterial inhibition rate of product which analyzed using the Mann Whitney U test.
 Results: Probiotic product inhibited growth of L. monocytogenes by (86 %), Y. pseudotuberculosis (82%), S. aureus (76%), E. coli (74%), S. Typhimurium (73%), P. aeruginosa (69%) and S. epidermidis (67%) respectively. The lowest antagonistic effect was detected against E. faecalis 44%. While probiotic product provided significant growth inhibition on 8 strains (p0.05).
 Conclusion: It was concluded that antagonistic effect of probiotic food supplement on test bacteria. Therefore it may be beneficial to use in bacterial infections.
Highlights
The gastrointestinal system hosts a microbial community called microbiota which is so important for health[1]
Test product containing a mixture of probiotic bacteria did not form an inhibition zone in two different bacterial strains, while an inhibition zone was found in others
Antimicrobial agent resistance caused by the consumption too much antibiotics and synthetic products leads to the need for natural products to protect against infectious diseases
Summary
The gastrointestinal system hosts a microbial community called microbiota which is so important for health[1]. The preservation of the natural structure of the microbiota is considered to be very important for human health[5]. Microbiota with dysbiosis, aside from the metabolic diseases it causes, directly leads to serious health problems such as constipation, diarrhea, other intestinal disorders and even colitis[6]. One of the important issues in preserving the natural structure of the microbiota is the presence of probiotics. Living microorganisms that live in the microbiota and are resistant to stomach acids and bile, which have significant beneficial effects on the host, are generally called probiotics[7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
