Investigation of amino acids in wolfberry fruit (Lycium barbarum) by solid-phase extraction and liquid chromatography with precolumn derivatization

Abstract

The aim of this work is to present a simple and sensitive reverse-phase liquid chromatography method for the determination and investigation of amino acids in wolfberry fruit (Lycium barbarum) after solid-phase extraction-derivatization. The method linearity, calculated for each amino acid, had a correlation coefficient higher than 0.9950, in concentrations ranged from 9.60 to 3.33×103μmolL−1. The detection limits of amino acids were between 2.42 and 6.51μmolL−1. The calculated recoveries for amino acids in wolfberry fruit were from 87.3% to 97.1% and relative standard deviations were from 2.62% to 5.22%. The investigation illustrated that each tested wolfberry fruit contained at least 16 amino acids and the main amino acids were glutamic acid, aspartic acid, proline, alanine, serine, glycine, lysine, and tyrosine. The levels of amino acid in wolfberry fruit were varying from different areas.