Investigation of Active Anti-Inflammatory Constituents of Essential Oil from Pinus koraiensis (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells.

Jiyoon Yang,Ki-Joong Kim,Chang-Deuk Eom,Won-Sil Choi,Mi-Jin Park

doi:10.3390/biom11060817

Jiyoon Yang, Ki-Joong Kim + Show 3 more

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https://doi.org/10.3390/biom11060817

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Journal: Biomolecules	Publication Date: May 31, 2021
Citations: 18	License type: CC BY 4.0

Affiliation: Korea Forest Service, Seoul National University

Abstract

In a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (Pinus koraiensis, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of P. koraiensis oil. The essential oil was extracted from P. koraiensis wood by hydrodistillation and was divided into six fractions (A–F) through fractional distillation. Then, the anti-inflammatory activities of the fractions (A–F) were determined. Fractions A and F markedly downregulated the production of pro-inflammatory cytokines as well as the secretion of β-hexosaminidase in lipopolysaccharide (LPS)-stimulated RBL-2H3 cells. The main constituents of the active anti-inflammatory A and F fractions were (+)-α-pinene, (−)-β-pinene, (+)-α-terpineol, 3-carene, (+)-limonene, and longifolene. These six single compounds decreased the expression of inflammatory-related genes (i.e., IL-4 and IL-13) as well as the secretion of β-hexosaminidase in LPS-stimulated RBL-2H3 cells. (+)-α-Pinene, (−)-β-pinene, (+)-α-terpineol, and longifolene exhibited the strongest effects; these effects were comparable to those of the positive control (i.e., dexamethasone). The findings indicate that the interactions between these components exhibit potential for the management and/or treatment of inflammatory conditions as well as base structures for the development of novel anti-inflammatory drugs.

Highlights

The Korean pine (Pinus koraiensis Siebold and Zuccarini) grows in Russia, Korea, China, and Japan at locations higher than 1000 m above sea level
The P. koraiensis oil showed an inhibitory effect on the β-hexosaminidase release, but the effect was insignificant compared with other oils. These results suggest that the essential oil had immunostimulatory effects on T cells and meaningfully inhibited allergy-associated cytokines IL-4 and IL-13
P. koraiensis wood oil was divided into six fractions (A–F) using fractional distillation

Summary

Introduction

The Korean pine (Pinus koraiensis Siebold and Zuccarini) grows in Russia, Korea, China, and Japan at locations higher than 1000 m above sea level. It can reach 1 m in diameter and 20–30 m in height [1]. The lignum of P. koraiensis is hard and has been widely used in North Asia and Korea as a furniture material for a long time. P. koraiensis contain high-quality fatty acids, amino acids, carbohydrates, and vitamins [2]. P. koraiensis has been used as a food supplement and in traditional Asian medicine for thousands of years. Studies have reported that the extract of P. koraiensis bark exhibits antitumor, antioxidant, antiaging, and antimutagenic activities.

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