Investigation of ACC-Deaminase and Indole Acetic Acid Producing Bacteria from Rhizospheric Soils in Ağrı Province

Burak Alaylar,Mehmet Karadayi

doi:10.21597/jist.854685

Abstract

Plant growth promoting rhizobacteria (PGPR) have critical role in promoting plant growth and health with various mechanisms. Production of indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity are well-known plant growth promoting (PGP) properties. In the current study; A total of 48 morphologically distinct bacterial colonies were chosen during the isolation of bacteria from various rhizospheric wheat, maize and sainfoin soil samples from agricultural areas in Ağrı-Turkey. The bacteria were isolated from soil samples utilizing by culture-dependent techniques. Then, the strains were visualised for PGP activities such as production acetic acid IAA and ACC deaminase activity. The 16S rRNA sequence similarity of potential PGPR rhizospheric strains demonstrated that strains belong to species Bacillus, Pseudomonas, Microbacterium, Pseudarthrobacter, Arthrobacter and Enterobacter. Totally, 11 of 48 isolates exhibited highly IAA producing and ACC deaminase activity. These results show that IAA producing and ACC deaminase activity of local potential PGPR strains isolated from soil rhizospheric which can appropriate for sustainable agricultural applications in Ağrı province.

Highlights

Plant–microbe interactions have beneficial processes plenty of key roles in soil health, crop growth and productivity
11 of 48 isolates exhibited highly indole acetic acid (IAA) producing and ACC deaminase activity. These results show that IAA producing and ACC deaminase activity of local potential Plant growth promoting rhizobacteria (PGPR) strains isolated from soil rhizospheric which can appropriate for sustainable agricultural applications in Ağrı province
In this regards; It can be understood from the present study that rhizospheric bacteria isolated from agricultural areas have crucial plant growth promoting (PGP) such as IAA production and ACC deaminase activity

Summary

Introduction

Plant–microbe interactions have beneficial processes plenty of key roles in soil health, crop growth and productivity. Soil microorganisms demonstrated positive effects on numerous agricultural applications. Among these soil microorganisms, plant growth promoting rhizobacteria (PGPR) was initially described as; soil bacteria that located into roots of plants and they have fruitful features when inoculation onto seed and improve to plant growth by Kloepper and Schroth (1978). For example; atmospheric nitrogen (N2) fixation, solubilization of phosphorus, improved to solubility of nutrients like synthesizing of siderophores, production of plant growth regulators such as cytokinins, ethylene, gibberellic acid, and indole acetic acid (IAA), and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity (Ahmad et al, 2008; Alaylar et al, 2020b)

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