INVESTIGATION NON-ALKALOIDAL BIOLOGICAL ACTIVE COMPOUNDS OF DATURA INNOXIA

Abstract

The purpose of the investigation is to study non-alkaloidal biological active compounds of various organs, the antimicrobial activity of plant oil and ethanolic extracts of Datura innoxia Mill. from nightshades (Solanaceae). D. innoxia grows in the natural environmental conditions of the Republic of Azerbaijan. We have studied presence of several biological active compounds – triterpenoic acids, essential oils, fatty acids, sterols and amino acids in the content of various organs of plant. Oil was obtained by using Soxhlet apparatus and investigated by GC method. After removing the oil from the seeds, triterpenoic acids - oleanolic and ursolic acids were isolated and identified by TLC. Essential oils were obtained from plant leaves by hydrodistillation and studied by GC-MS method. 20 amino acids were identified in the aerial part of the plant by the HPLC method, with amino acid analyzer L-8800 (Hitachi, Ltd.), 12 of them are non-essential, 8 are essential.
 The antimicrobial activity of plant oils of seed and ethanolic extracts of some parts (roots, stems, leaves, flowers, fruits and seeds) were studied on Staphylococcus aureus, Esherichia coli, Pseudomonas aeruginosa, Bacillus anthracoides, Klebsiella pneumoniae and Candida albicans. The significant activity of some extracts (stems, leaves, fruits and seeds) was observed against S. aureus, E. coli, C. albicans, B. anthracoides and plant oil against C. albicans.