Abstract
Research has established that the most significant mechanism associated with tobacco-specific nitrosamine (TSNA) formation in flue-cured tobacco results from exposure to nitrogen oxides (NOx) that react with the naturally occurring alkaloids in the green leaf during curing. As a result, the tobacco industry initiated a conversion in 1999 from direct-fired to indirect-fired heating systems in the curing barns. This work began with random monitoring of direct- and indirect-fired curing barns to quantify the nitrogen oxide (NOx) concentrations to which the green leaf is exposed during the curing process. Varying concentrations of both nitric oxide (NO) and nitrogen dioxide (NO2) were measured. Controlled exposure tests were conducted during the 2004 and 2005 growing seasons to evaluate any differences in the cured-leaf TSNA level as a result of exposure to NO or NO2 during curing. Two electric-heated curing barns were utilized to perform the exposure study. One barn was dosed with a NOx gas species and the a...
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