Abstract
Background and aimTreatment of burn wound infections has become a global challenge due to the spread of multidrug-resistant bacteria; therefore, the development of new treatment options for the mentioned infections is essential. Platelets have drawn much attention for this purpose because they are a safe and cost-effective source of different antimicrobial peptides and growth factors. The present study evaluated antibacterial effects and wound healing properties of Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections.MethodsPdB was prepared through the freezing and thawing process and then, in vitro antibacterial effect was determined by disk diffusion and broth microdilution methods. Afterward, burn wound was inflicted on 56 rats, infected with both bacteria, and topical administration was performed to evaluate antibacterial effects and wound healing properties of PdB.ResultsIn vitro results showed that PdB inhibited the growth of A. baumannii in the highest dose (0.5), while we did not detect any inhibitory effects against K. pneumoniae. By contrast, PdB significantly inhibited the growth of bacteria in treated animal wounds compared to the control groups (P value < 0.05). Macroscopic assessments pointed to the significant enhancement of wound closure in the treated animals. In addition, histopathological examination demonstrated that treatment of rats with PdB led to a considerable increase in re-epithelialization and attenuated the formation of granulation tissue (P value < 0.05).ConclusionThe use of topical PdB is an attractive strategy for treating A. baumannii and K. pneumoniae burn wound infections because it inhibits bacterial growth and promotes wound healing properties.
Highlights
Burn is identified as the fourth most common type of trauma worldwide that injures body tissues, especially skin [1]
In vitro antibacterial activity of F‐Platelet-derived Biomaterial (PdB) F-PdB had an inhibitory effect against A. baumannii in Disk Diffusion (DD) and broth microdilution methods
The results of using broth microdilution methods showed that F-PdB inhibited the growth of A. baumannii at the highest concentration (0.5)
Summary
Burn is identified as the fourth most common type of trauma worldwide that injures body tissues, especially skin [1]. Burn injuries lead to prolonged hospitalization and such patients are more likely to be infected with nosocomial pathogens. There is an increased risk of developing an invasive burn wound infection for burn patients such that bacteremia and multiple-organ dysfunction syndrome will develop. In this regard, burn wound infections, directly or indirectly, cause death by 33 to 80% [3, 4]. Many factors like breakdown of the skin barrier, immunodeficiency, inter-institutional transfer, and invasive procedures carried out at healthcare facilities make burn patients more susceptible to infections. Platelets have drawn much attention for this purpose because they are a safe and cost-effective source of different antimicrobial peptides and growth factors. The present study evaluated antibacterial effects and wound healing properties of Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Annals of Clinical Microbiology and Antimicrobials
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.