Investigation for Cryptosporidium spp. oocysts of drinking water in Van

Abstract

Objective: There are many microorganisms transmitted by drinking water and causing epidemic diseases in humans. One of these, Cryptosporidium spp is a protozoon causing enteritis transmitted with contaminated waters. Oocysts of the parasite thrown with human and animal feces cause contamination of drinking water sources in environments with poor sanitation. Oocysts are resistant to the environmental conditions and disinfectants concentrations which are effective on bacteria. Oocysts rise to important problems in water treatment due to remaining alive for a long time in water and the resistance to disinfectants. This study aimed to determine the prevalence of this parasite in the drinking water of the Van region. Method: Drinking water samples were taken from 440 sources totally. Water samples were brought to laboratory in five liter clean plastic bins and filtrated from filtration device with vacuum pump which has 0.45 μm cellulose acetate membranes. Particles on the filter were centrifuged by washing in 20 ml of the same water sample, and the sediment was left on the slide. Prepared slides were examined by staining with modified acid fast method after filtering through filtration devices. Three stained slides were prepared for each sample. Results: Cryptosporidium oocysts were detected in 1.13% of the total 440 water samples. Oocysts were determined in 1.57% of 191 surface water sources and OZET Amac: Icme suyu ile bulasan ve insanlarda salgin hastaliklara sebep olan bircok mikroorganizma vardir. Bunlardan biri olan Cryptosporidium spp. kontamine sularla bulasarak enterite neden olan bir protozoondur. Insan ve hayvan diskisiyla atilan bu parazitin ookistleri sanitasyonu kotu cevrelerde icme suyu kaynaklarinin kontaminasyonuna yol acmaktadir. Ookistler cevre sartlarina ve bakterileri etkisiz hale getirecek konsantrasyondaki dezenfektanlara direnclidir. Su ortaminda uzun sure canli kalabilmeleri ve dezenfeksiyona dayanikli olmalari su aritma islemlerinde onemli sorunlar olusturmaktadir. Bu calismada Cryptosporidium’un yoremiz icme sularindaki yayginliginin belirlenmesi amaclanmistir. Yontem: Icme suyu olarak kullanilan toplam 440 kaynaktan su ornekleri alinmis ve beser litrelik temiz plastik bidonlara alinarak laboratuvara getirilmistir. Su ornekleri 0,45 μm’lik seluloz asetat membran filtresi bulunan vakum pompali filtrasyon cihazindan suzulmustur. Filtre uzerindeki partikulat ayni su orneginin 20 ml’si icinde yikanarak santrifuj edilmis ve cokelti lam uzerine birakilmistir. Hazirlanan preparatlar filtrasyon cihazindan suzuldukten sonra modifiye asit-fast yontemiyle boyanarak incelenmistir. Her bir ornek icin ucer preparat hazirlanmistir. Bulgular: Toplam 440 su orneginin %1,13’unde Cryptosporidium spp. ookistleri saptanmistir. Icme suyu olarak kullanilan 191 yuzeysel kaynak suyunun 1 Yuzuncu Yil Universitesi, Tip Fakultesi, Tibbi Parazitoloji Ana Bilim Dali, VAN 2 Van Il Saglik Mudurlugu, Halk Sagligi Laboratuvari, VAN Gelis Tarihi / Received : Kabul Tarihi / Accepted : Iletisim / Corresponding Author : Mutalip CICEK Yuzuncu Yil Universitesi, Tip Fakultesi, Tibbi Parazitoloji Ana Bilim Dali, VAN Tel : +90 412 248 80 01/4092 E-posta / E-mail : muttalipcicek@hotmail.com 29.05.2011 23.07.2011 DOI ID : 10.5505/TurkHijyen.2011.26214 Turk Hijyen ve Deneysel Biyoloji Dergisi Cicek M, Korkoca H, Akkas O. Van Ili icme sularinin Cryptosporidium spp. ookistleri yonunden incelenmesi. Turk Hij Den Biyol Derg, 2011; 68 (3): 122-6.