Abstract
Although our understanding of DNA methylation mechanisms and functions has vastly improved over the past two decades, the entire spectrum of influences of this potent mechanism is not fully grasped yet. Today, a wide variety of techniques are used to examine DNA methylation patterns but most of these methods are still relatively expensive and many are platform specific (Prokhortchouk and Defossez, 2008). The need has arisen to develop an economically viable and uncomplicated technique for DNA methylation analysis. The comet assay (single cell gel electrophoresis or SCGE) is a cost-effective, sensitive and simple technique which is traditionally used for analysing and quantifying DNA damage in individual cells (Azqueta, et al., 2011, Fairbairn, et al., 1995). By modifying this assay to be methylation sensitive, global DNA methylation can be routinely measured in cell cultures while simultaneously being able to deduce the integrity of the genetic material of the examined cells. The methylation sensitive comet assay was used to examine the effect of the accumulating metabolite, succinylacetone (SA), in hereditary Tyrosinemia type I (HT1), since hepatocellular carcinoma (HCC) frequently develops into this disease.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
