Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats.

Abstract

The current study aimed to investigate the protective effects of moderate aerobic exercise against chlorpyrifos (CPF)-induced testes dysfunction. In excremental study, 48 adult male albino rats were randomly allocated into 16 groups of 3 rats each. Twelve experimental groups received intraperitoneal injection (5days a week) of either 1.0 or 3.0mg/kg body weight CPF in DMSO for 2, 4 or 6 consecutive weeks. Seven of these experimental groups were subjected to run at moderate exercise intensity for 5days per week over 2weeks, whereas the other groups were not. Two groups (sham groups) were administered to the equal volume of vehicle (DMSO) for 4 or 6 consecutive weeks. The remaining two groups comprised the control groups including a sedentary and an exercise-trained control group. Exercise training leads to a markedly increase in testicular superoxide dismutase (SOD) activity in CPF-exposed rats compared with corresponding sedentary animals (p<.05). Lipid peroxidation level was found to be significantly decreased in the testis of exercised animals that had been exposed to CPF (p<.05). Our results suggest that aerobic exercise can alleviate the oxidative stress induced by sub-acute CPF exposure in testis. Exercise training could barely mitigate CPF-induced testicular damages in rats.