Abstract
AbstractIn this study we examine the depth profiles of two bio‐materials, an amino acid (arginine) and a polypeptide (Gly‐Gly‐Tyr‐Arg), to observe the effects of temperature on sample analysis with time of flight secondary ion mass spectrometry (ToF‐SIMS). The samples were prepared as dried droplet films on silicon and were analyzed at room temperature (300 K) and at cryogenic temperatures (100 K). Under cryogenic analysis conditions, molecular ion yields at the steady state increased by factors of 1.8 and 5 for arginine and the tetrapeptide respectively compared to analysis at room temperature. Increases are also observed in arginine dimer and trimer ion formation by factors of 6 and 5 respectively, while the lower mass fragment ions investigated did not change in intensity by more than 30%. Further analysis of the results show that the decrease in the steady state ion yield with primary ion fluence at 300 K is reduced when analyzed at 100 K, indicating a decrease in chemical damage. These results suggest that analysis under cryogenic conditions provides several benefits for the analysis of biological compounds and hence for 3D molecular imaging of cells and tissue. Copyright © 2010 John Wiley & Sons, Ltd.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.