Investigating the effect of tannin films prepared by low-energy electron beam deposition on the inhibition of bone tumors

Abstract

Osteosarcoma is a prevalent malignant bone tumor and incomplete surgical resection of the tumor often results in higher rates of local recurrence. To tackle this issue, there is an urgent requirement to develop implants that possess the capability to inhibit tumor recurrence. In this paper, we prepared three different thicknesses of tannin (TA) films on titanium substrates using low-energy electron beam deposition to study their effects on bone tumor inhibition. The structure and morphology of TA films were analyzed by FT-IR, XPS, SEM, and EDX. The release kinetics of tannins were investigated, revealing their ability to achieve sustained release. This sustained release is advantageous for long-term inhibition of bone tumors. Cellular experiments, including cytotoxicity test, live/dead double staining, cell migration and western blot, were performed to evaluate the tumor-suppressing activity of TA films in vitro. The results indicated that the TA films were not only non-cytotoxic to normal bone cells, but also exhibited excellent in vitro anti-tumor activity by effectively inhibiting the activity and migration of bone tumor cells.