Investigating the biocompatibility, flexural strength, and surface roughness of denture base resin containing copper oxide nanoparticles: An in vitro study

Abstract

AimThis study aimed to evaluate the biocompatibility, flexural strength, and surface roughness of polymethyl methacrylate (PMMA) containing Copper Oxide Nanoparticles (CuO NPs) at different concentrations. Methods25 heat-polymerized PMMA wax patterns fabricated in 5 groups containing 0.5, 5, 50, and 500 μg/ml CuO NPs and nanoparticle (NP)-free PMMA discs were prepared. 5 growth mediums (DMEM with 10% FBS and 1% penicillin-streptomycin) without disks were also incubated similarly to serve as the control groups. The cytotoxicity of the discs was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on cultured Human Gingival Fibroblasts. The number of 1.3 × 104 cells were seeded in each well of 96-well plates (5 wells for the extract of each specimen). Days 1, 3, 5, and 7 were the intervals that the culture media were in direct contact with the PMMA discs for either 24 or 72 h. After that, a total of 40 specimens with 65 × 10 × 2.5 mm dimensions were prepared in five groups (n = 8). The specimens were subjected to a rugosimeter for the evaluation of surface roughness. The flexural strength test was performed using a universal testing machine. Microscopic evaluation was performed for the dispersion of the NPs. Non-parametric Kruskal-Wallis test and parametric one-way ANOVA test were used for data analysis. ResultsThe samples containing 500 μg/ml NPs showed the lowest percentage of cell viability at all incubation periods, while the highest cell viability was observed in NP-free PMMA 24 h after the seventh day of incubation. NPs at 50 and 500 μg/ml concentrations showed strongly significant differences in cytotoxicity compared to the 0 concentration and the control group (p < 0.001). Although all the samples demonstrated an increasing pattern of cell viability on the third, fifth, and seventh days, the percentage of cell viability was significantly lower after 72 h than after 24 h in all incubation periods (p < 0.001). NPs significantly increased flexural strength (p = 0.005) but did not affect the surface roughness of the PMMA discs (p = 0.396). SignificanceThe CuO NPs were cytotoxic only when applied in high concentrations, but presented a descending trend over time. No cytotoxic effect was observed in the experimental groups after seven days of incubation. Furthermore, CuO NPs increased flexural strength, but the surface roughness of the PMMA discs was not affected.