Abstract
Background: Curcumae Rhizoma (CR) comes from Curcuma genus, functional breaking blood stasis, detumescence and acesodyne for treatment of Zhengjia accumulation, amenorrhea, traumatic injury and bruising pain. Modern pharmacological studies have shown that the main monomer compositions of Curcumae Rhizoma, such as curcumol, β-elemene, curcumin, have good anti-tumor effects. However, the mechanisms are not clear yet. Previous studies have revealed that the associated glycoprotein showed significant differences with normal people after the development of hepatocellular carcinoma (HCC). Methods: In this study, the anticancer activities of CR extract were investigated by constructing HepG2 tumor bearing nude mice models. Furthermore, glycan profiles of tumor tissues were thoroughly characterized by lectin microarrays, a high-throughput technique, in an effort to explore the anticancer mechanism of Curcumae Rhizoma. Results: It is indicated that CR extract might inhibit the tumor proliferation in tumor-bearing model and the potential mechanisms might be CR treatment altered protein glycosylation of tumor cell, which plays an important role in the pathogenesis and progression of HCC. In detail, fucosylation (identified by PSA and UEA-I), bisecting GlcNAc or multianternnary (identified by PHA-E+L) and terminal GalNAc (identified by BPL) decreased, while sialylation (identified by WGA and SNA), high-mannose (identified by ConA) and T-antigen/TN-antigen/sialyl-T antigen (identified by ACA) increased in CR treatment group compared to model group. Similar phenomenon also occurred in two positive groups, western medicine cyclophosphamide (CTX) and Chinese medicine monomer β-elemene administration groups, especially in β-elemene administration one. The liver contains various receptors on sinusoidal and hepatocyte surfaces, and many proteins that bind to these receptors reply on carbohydrate moieties during the development of HCC. Conclusion: In this point of view, a search for the biological significance of glycosylation expression and its function after Chinese Medicine administration in HCC may open a new direction in glycobiology.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.