Investigating SnocCSP4 expression and key compound interactions with SnocOBP4 in Sirex noctilio Fabricius (Hymenoptera: Siricidae)

Abstract

Sirex noctilio, a significant pest impacting Pinus sylvestris var. mongolica, presents control difficulties due to its wood-boring behavior, paucity of natural antagonists, and wide-ranging habitats. Our research aims to elucidate the functionality and operational mechanisms of chemosensory proteins 4 (SnocCSP4), providing strategic insights for pest management and fostering further exploration in CSPs. Techniques such as qPCR, fluorescence binding affinity assays, molecular docking, and dynamic simulations were utilized to investigate the tissue-specific distribution, ligand binding capacities, and mechanistic underpinnings of SnocCSP4. The findings revealed a high abundance of SnocCSP4 in male genitalia, significant sexual dimorphism in its expression, and high binding affinities to (−)-Globulol and 10-Oxodecanoic acid. Subsequent analysis identified hydrophobic cavities formed by non-polar amino acids (VAL, LEU, ILE, LYS) and the critical role of polar amino acids (ALA 46, GLU 45, THR 75) in maintaining system stability. These insights suggest the primary role of SnocCSP4 in binding or transporting these volatiles and indicate that modifying key amino acids could inform the design of more effective pest control measures.