Abstract
The adoption of axially oriented line illumination patterns for fluorescence excitation in small animals for fluorescence surface imaging (FSI) and fluorescence optical tomography (FOT) is being investigated. A trimodal single-photon-emission-computed-tomography/computed-tomography/optical-tomography (SPECT-CT-OT) small animal imaging system is being modified for employment of point- and line-laser excitation sources. These sources can be arbitrarily positioned around the imaged object. The line source is set to illuminate the object along its entire axial direction. Comparative evaluation of point and line illumination patterns for FSI and FOT is provided involving phantom as well as mouse data. Given the trimodal setup, CT data are used to guide the optical approaches by providing boundary information. Furthermore, FOT results are also being compared to SPECT. Results show that line-laser illumination yields a larger axial field of view (FOV) in FSI mode, hence faster data acquisition, and practically acceptable FOT reconstruction throughout the whole animal. Also, superimposed SPECT and FOT data provide additional information on similarities as well as differences in the distribution and uptake of both probe types. Fused CT data enhance further the anatomical localization of the tracer distribution in vivo. The feasibility of line-laser excitation for three-dimensional fluorescence imaging and tomography is demonstrated for initiating further research, however, not with the intention to replace one by the other.
Highlights
Resolved detection of fluorescence-labeled agent distribution in mice has been demonstrated to hold great potential in a broad spectrum of preclinical molecular imaging studies.[1]
The line laser beam is aligned with the axial long axis of the bore, as well
Because of the extended excitation pattern throughout the axial direction generated by the line laser, the approximation of Downloaded From: https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics on 08 Nov 2021 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use
Summary
Resolved detection of fluorescence-labeled agent distribution in mice has been demonstrated to hold great potential in a broad spectrum of preclinical molecular imaging studies.[1]. Noncontact data acquisition approaches have replaced fiberbased contact imaging implementations for the most part mainly because of animal handling and efficiency shortcomings of the latter.[5] Some noncontact imaging systems perform fluorescence optical tomography (FOT) reconstruction by slightly squeezing the imaged animal into, or between, a purposely shaped chamber of simple geometry (e.g., translucent vices),[6,7] or within translucent compartments of (generally) cylindrical geometry with lesser spacing constraints forced onto the animal.[8,9] Planar spacing by the use of opposite vices yields a much reduced boundary complexity involved in solving the inverse problem. Fluorescence surface imaging (FSI) is performed by illuminating the entire imaged object, or local regions thereof, with a large, preferably homogeneous field of light to excite fluorochromes in a large area, generally the whole animal
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