Investigating dual drug loaded PLGA nanocarriers for improved efficacy in endometritis therapeutics

Abstract

Modern endometritis therapeutics may require an extremely precise and controlled drug release system. Poly lactic-co-glycolic acid (PLGA) microspheres loaded with two different drugs like amoxicillin and vancomycin were prepared and their efficacy in management of endometritis in murine model was examined. Such biological nanocarriers are need of the hour since they are biodegradable, cytocompatible, hemocompatible and easily formulated. The sizes of the drug loaded PLGA microspheres ranged from 65–100 nm. The electron microscopy images depicted them as uniform spherical structures. The zeta potential and polydispersity index were calculated and the in vitro release investigation indicated steady drug release over a period of 50 h at effective dosage. The drug loaded nanocarriers were found with be cytocompatible and hemolysis test elaborated that they met with hemolysis rate safety requirements. The murine models were induced with endometritis by intravaginal administration of bovine uterine isolates. The mice were sacrificed and histopathogical examination of the endometrial slides stained with haematoxylin and eosin revealed that the drug loaded PLGA nanospheres helped in reducing acute endometritis in mice in a very short time. This study thereby demonstrated well described synthesis approach of drug loaded PLGA nanospheres which may be further be transferred to preclinical laboratory studies.