Abstract
Due to public apprehension regarding the use of chemical preservatives to prevent food spoilage and food-borne diseases, it is imperative to identify natural alternatives such as antimicrobial peptides as a potential solution. The study aimed at evaluating the effectiveness of the antimicrobial peptide RI12 (K3W) against Listeria monocytogenes. RI12 (K3W) exhibited potent antimicrobial properties, with a minimum inhibitory concentration and minimum bactericidal concentration of 16µM and 32µM, respectively. The time-kill assay revealed a consistent reduction in bacterial viability at 8, 16, and 24h of study. Cytotoxicity testing on mammalian cells demonstrated no apparent change in morphology or cell count. Investigating how well it worked in a food matrix to replicate real-world conditions showed a significant decrease in the bacterial count. The study underscores the potential of RI12 (K3W) as a safe and effective antimicrobial against L. monocytogenes that might also serve as an alternative to chemical preservatives.
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