Investigação dos níveis da proteína S100β (técnica de Western Blot) no líquor de cães com extrusão de disco intervertebral tóraco-lombar submetidos à eletroacupuntura

Abstract

HAYASHI, A. M. S100β Western blotting searching in cerebrospinal fluid of dogs with thoracolumbar intervertebral disk disease submitted by electroacupuncture treatment. [Investigacao dos niveis da proteina S100β (tecnica de Western blot) no liquor de caes com extrusao de disco intervertebral toraco-lombar submetidos a eletroacupuntura]. 2010. 109 f. Tese (Doutorado em Ciencias) – Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo, Sao Paulo, 2010. Electroacupuncture treatment in dogs with thoracolumbar disk disease has the purpose of analgesia, motor and sensorial rehabilitation. The functional improvements observed after spinal cord lesion are resulting of synaptic plasticity. This fact is a result of compensatory mechanisms and not structural repair. S100β is considered a glial neurotrophic factor. It can be involved with these improvements. A prospective clinical study during February 2007 to June 2008 evaluated electroacupuncture (EA) treatment (group EA) in dogs with thoracolumbar disk extrusion (n=10) without corticosteroids. Cerebrospinal fluids (CSF) were collected from these animals in two time points: during the mielography and/or computed tomography (M1) and after 35.5±21.33 (Mean±SD) days after EA treatment (M2). S100β levels in CSF were investigated in dogs of group EA by Western blot. CSF of dogs without neurological signs (group normal; n=7) were used for comparisons. For the statistical analysis, significant level of 5%, ANOVA for repeated and not repeated variables (post-test Bonferroni s), Student-t test for two variables comparisons and Pearson’s linear correlation for quantitative variables, were used. All dogs (group EA) with lesion levels of dysfunction 3 to 5, reached neurological improvements with significant higher scores (P-value 10 (Pvalue=0.520) and between group of dogs with number of lesions scores 1, 2, 3 and 4 (Pvalue=0.5262). S100β levels at M2 have no correlation with duration of clinical signs (r=0.0304; Pvalue=0.629), extrusion length (r=0.0158; P-value=0.729), however there was a tendency of correlation with time of ambulation return (r=0.3852; Pvalue=0.055). Electroacupuncture can modulate the S100β levels in CSF of dogs with thoracolumbar disk extrusion. Electroacupuncture up-regulated S100β in CSF of dogs with late motor rehabilitation. This fact suggests its contribution in the neural plasticity after spinal cord lesion due to intervertebral disk extrusion.