Abstract
A systematic investigation of the growth mode and appearance of two cell wall enzymes (invertase E.C. 3.1.1.26, and acid phosphatase E.C.3.1.3.2) in free and immobilized cells of Saccharomyces cerevisiae was carried out. Gel-entrapped yeast cells were stimulated into growth by incubation in media differing from one another in composition, under different experimental conditions. In immobilized cells, lower growth rates were observed which were mainly due to the resistance of the matrix to nutrient diffusion. Invertase activity reached levels higher than those of corresponding free cells. This result was independent of the cultural system adopted for growth and of the nature of the gel matrix chosen for immobilization, and could not be explained merely in terms of diffusional resistance. A possible explanation could be some physiological change related to the state of immobilization of the yeast cells. What was observed with invertase seems to be peculiar to this enzyme, as the extent of repression, in the case of acid phosphatase, was almost the same for both free and gel-immobilized cells.
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