Invertase adsorption with polymers functionalized by aspartic acid

Abstract

Abstract Today, the separation and purification processes are highly preferred over the affinity interactions in the scientific world. Among the materials used for this purpose, magnetic particles and cryogels are very popular. Both polymeric structures have their advantages and disadvantages. In this study, poly(2-Hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid), poly(HEMA-MAsp), magnetic microparticles, and cryogels were synthesized, and adsorption performances of both polymeric structures were investigated by using invertase from aqueous systems. Invertase (β-fructofuranoside fructohydrolase, EC 3.2.1.26) is a commercially important enzyme used in the food industry to obtain the product called invert sugar, which consists of a mixture of equivalent amounts of glucose and fructose. Therefore, it was preferred as a model enzyme in adsorption studies of polymeric structures. According to the results, 104.1 mg g−1 and 135.5 mg g−1 of adsorption capacity values were obtained for cryogel and magnetic microparticle forms, respectively. Increasing temperature slightly reduced the adsorption capacity of both polymeric structures. In the adsorption/desorption cycle studies performed five times with poly(HEMA-MAsp) polymers, both forms were found to have high reusable properties. It was determined that the activity of invertase immobilized on polymeric structures was preserved at a rate of 83.6% for the particle form and 89.2% for the cryogel form.