Abstract
The expression of nm23-H1 mRNA and protein was studied in colorectal cancers by Northern blotting and immunohistochemistry. All 21 colorectal cancers studied by Northern blotting had increased levels of nm23-H1 mRNA relative to the adjacent normal colonic mucosa. Increased nm23-H1 protein expression was also observed in all 36 colorectal cancer cases including those studied by Northern blotting. There was no significant correlation between nm23-H1 expression and tumour histology, serosal invasion, lymphatic invasion, venous invasion, or lymph node metastasis. However, the expression of both mRNA and protein was significantly lower in tumours associated with liver metastasis than in those without such metastasis. These observations indicate that the nm23 gene may play a role in the suppression of liver metastasis of colorectal cancer.
Highlights
Liver metastasis was found in nine patients (25.0%), including one whose liver was free of metastasis at the time of operation, and subsequently became metastasis positive
The expression of nm23-HI by the colorectal cancers exceeded that of the adjacent normal colonic mucosa in all 21 patients for whom we analysed paired tissue samples, even after correction with
The expression of nm23-H1 protein in colorectal cancers and the adjacent normal mucosa was analysed by immunoblotting using monoclonal antibody (mAb) HI-229, which is specific for human nm23-Hl protein
Summary
Patients and tissue samples Specimens of 36 colorectal cancers and the adjacent normal mucosa were obtained from patients operated at the Second Department of Surgery, Kanazawa University School of Medicine, between 1989 and 1992. They included 21 patients with colon cancer and 15 with rectal cancer. Probes were labelled with [a-32P]dCTP using a multiprime labelling kit (Amersham). The BamHI-EcoRI fragment of pBSK-H1 was used as the probe (Urano et al, 1992a). Levels of expression of the P-actin were employed as an internal standard to correct the compared samples for variations in the amount of messenger RNA loaded
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