Invention of a fast response biosensor based on Au-PolyPyrrole nanocomposite-modified quartz crystal to detect morphine concentration

Abstract

A biosensor based on a 20 MHz oscillator was designed by modifying the quartz surface with the Au nanoparticle in PolyPyrrole (AuNP-PPy) layer. In this design, a new field programmable gate array board was used. The AuNP-PPy composite was layered on a quartz surface with 5 μm thick using centrifugal force as simple method. Biosensor components were characterized by FESEM, XRD and FTIR spectrum. The recording of the frequency of the biosensors before the detection of morphine showed that the syntheses were the same, the frequency of all the sensors were almost equal and the experiments were repeatable. The biosensor was stable up to 50 °C but performed better at 28 °C. The limit of morphine concentration detection by biosensor was 0.1 ngml-1. Detection of morphine concentration in unknown samples showed a detection error of less than 4%. Regeneration results showed that this biosensor can be used up to 8 times in a row.