Invasive microstegium vimineum (Japanese stiltgrass) hinders growth and biomass of hardwood seedlings regardless of light and moisture regime

Abstract

Limited natural oak (Quercus spp.) regeneration in current-day, closed-canopy upland oak forests across the eastern and central United States has prompted increased use of various silvicultural practices, including prescribed fire and/or thinning, to reduce competition from shade-tolerant, often fire-sensitive species, and ultimately, restore and/or maintain oak landscapes. However, these practices create disturbances that can promote the spread of invasive plants and complicate oak restoration attempts, especially if invasion differentially impacts oaks compared to competitor species during early seedling development. To evaluate if Japanese stiltgrass (Microstegium vimineum (Trin.) A. Camus), an invasive C4 grass common in the understory of these systems, impacts growth and biomass of hardwood seedlings in different light and moisture environments, we established a shadehouse experiment in May 2020 with one-year old seedlings of white oak (Quercus alba L.) and two competitor species, red maple (Acer rubrum L.) and sweetgum (Liquidambar styraciflua L.), in the following treatments: M. vimineum (MV) presence (with/without), light (40/10 % full irradiance), soil moisture (high/low), and their interactions. Monthly, from May to October 2020, we measured seedling height growth, stem basal diameter, and MV cover and height if present. In October 2020, we harvested a subset of seedlings and measured their above- and belowground biomass and aboveground MV biomass when present. At study cessation, regardless of light or moisture treatment, red maple and sweetgum were significantly shorter when grown with MV (17.9 and 15.6 cm, respectively) than without MV (33.5 and 22.3 cm, respectively) and had smaller basal diameters (with MV: 1.03 and 0.94, respectively; without MV: 1.34 and 1.25 cm, respectively) and ∼ two times less biomass. White oak basal diameter was 1.2 times lower with MV (0.77 cm) compared to without MV (0.93 cm), but MV had no impact on white oak height growth or biomass, likely because of its conservative growth strategy and high-water use efficiency. Light and moisture treatments had little impact on MV height and cover, but MV biomass was greater with high (462 dry wt m−2) compared to low (398 g dry wt m−2) moisture treatment and ∼ 1.3 times greater when grown without vs with tree competition. Thus, MV invasion into closed-canopy upland oak forests may have species-specific effects on early seedling growth, reducing growth of competitor seedlings with non-conservative growth strategies more than that of upland oaks, but long-term studies are needed to better understand the potential legacy effects of MV on growing conditions and tree regeneration.