Invasion of Ureaplasma diversum in Hep-2 cells

Lucas Miranda Marques,Beatriz A Cortez,Renata L Neto,Priscilla M Ueno,Maurício Yamaguti,Ana Márcia S Guimarães,Melissa Buzinhani,Telma A Monezi,Gláucia M Machado-Santelli,Antonio Carlos R Braga,Jorge Timenetsky,Rosângela C Oliveira

doi:10.1186/1471-2180-10-83

Lucas Miranda Marques, Beatriz A Cortez + Show 10 more

Open Access

https://doi.org/10.1186/1471-2180-10-83

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Abstract

BackgroundUnderstanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay.ResultsThe isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma.ConclusionsThe results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.

Highlights

Understanding mollicutes is challenging due to their variety and relationship with host cells
The use of non-phagocytic cells to study mollicutes has been of great interest mainly since Mycoplasma fermentans was initially considered a cofactor in the pathogenesis of AIDS [5]
U. diversum adhesion and invasion on Hep-2 cells observed by Confocal Laser Scanning Microscopy (CLSM) The images of infected cells were from the apical surface to the basolateral region and differentiated the actin filaments in green, from the blue luminescence of nuclei

Summary

Introduction

Understanding mollicutes is challenging due to their variety and relationship with host cells. This contamination affects research by invalidating results in diagnosis Interference by these bacteria in mammalian non phagocytic cell cultures has been used to study mollicute biology [2]. The opportunism of Mollicutes is a challenging subject These microbes are diverse enough to explain their relationship variety with the host cells [3]. The use of non-phagocytic cells to study mollicutes has been of great interest mainly since Mycoplasma fermentans was initially considered a cofactor in the pathogenesis of AIDS [5]. Other mycoplasmas showed this same characteristic when inoculated in Ureaplasma diversum is a bovine-originated mollicute, first isolated in 1969 and considered a non-pathogenic species. Little is known about the virulence and pathogenic mechanisms of this mollicute

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