Invasion by Cenchrus spinifex changes the soil microbial community structure in a sandy grassland ecosystem

Abstract

Continuous nitrogen deposition increases the nitrogen content of terrestrial ecosystems and alters the soil nitrogen cycling process. Invasive plants have strong environmental adaptability, which can not only affect the composition and diversity of soil microbial community but also significantly affect the transformation process of soil nitrogen, leading to successful invasion. Currently, research on invasive plant soil ecosystems mainly focused on changes in soil nutrients and soil microorganisms. As an invasive annual grass weed with strong ecological adaptability, the impact of Cenchrus spinifex at different growth periods on soil environment and soil microbial structure composition and diversity in sandy grassland ecosystems is still unclear. In this study, soil samples were collected from four habitats with different degrees of invasion in situ during the vegetation and reproductive growth periods of Cenchrus spinifex. High-throughput sequencing and qPCR technology were used to analyze the changes in the composition, structure and diversity characteristics of the soil microbial communities during Cenchrus spinifex invasion. The results indicated that Cenchrus spinifex invasion had different effects on the soil environment at different growth periods, and Cenchrus spinifex had a preference for the utilization of ammonium nitrogen during vegetation growth period. Moreover, Cenchrus spinifex invasion significantly changed the composition and structure of soil bacterial communities, and the response of soil bacterial and fungal communities to the invasion was inconsistent. Additionally, the bacterial network was more stable than the fungal network. At different growth periods, Cenchrus spinifex had a significant impact on the key microbial communities of soil nitrogen cycling. The invasion increased the abundance of nifH and AOA-amoA, while decreased the abundance of AOA-amoB. Alkaline hydrolyzed nitrogen, total nitrogen and total phosphorus content were key factors that affect vegetation growth period and change the key microbial communities of nitrogen cycling. Alkaline hydrolyzed nitrogen, total phosphorus and organic carbon were key factors in reproductive growth period that alter the nitrogen cycling of key microbial communities.